Vaccinia E5 is a major inhibitor of the DNA sensor cGAS
Yang, N., Wang, Y., Dai, P., Li, T., Zierhut, C., Tan, A., Zhang, T., Pan, H., Li, Z., Ordureau, A., Xiang, J.Z., Hendrickson, R.C., Funabiki, H., Chen, Z., and Deng, L.
bioRxiv 2021.10.25.465197 (2021)
In this study, we identify an inhibitor of cGAS encoded by Vaccinia virus.
Structural basis for the inhibition of cGAS by nucleosomes
Kujirai, T.*, Zierhut, C.*, Takizawa, Y., Kim, R., Negishi, L., Uruma, N., Hirai, S., Funabiki, H., and Kurumizaka, H.
In this study, using cryo-EM and in vitro reconstitution, we present the mechanistic basis for the inhibition of cGAS by nucleosomes.
Regulation and Consequences of Self-DNA recognition by cGAS.
Zierhut, C.**, and Funabiki, H
Trends Cell Biol, 30 (2020), 594-605
In this review, we discuss how cGAS can be activated by self-DNA, and what is known about the ensuing cellular consequences.
The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death
Zierhut, C.**, Yamaguchi, N., Paredes, M., Luo, J.D., Carroll, T., and Funabiki, H.**
In this study, we show that the innate immune sensor of pathogenic DNA, cGAS, is suppressed on chromatin, but activated under aberrant conditions such as mitotic arrest, where it promotes apoptosis.
Nucleosome-Dependent Pathways That Control Mitotic Progression
Funabiki, H., Jenness, C. and Zierhut, C.
VCP/p97 extracts sterically trapped Ku70/80 rings from DNA in double strand break repair
van den Boom, J., Wolf, M., Weimann, L., Schulze, N., Li, F., Kaschani, F., Riemer, A., Zierhut, C., Kaiser, M., Iliakis, G., Funabiki, H., Meyer, H.